There are several methods that are still used with technology. We can go from chemical sterilization, through heat or even ultrasound. The most important thing is that you know how to select your most effective instrument sterilization method for the proper operation of your lab:
CHEMICAL METHODS: These methods cause the loss of viability of microorganisms. This can be achieved by using sodium hypochlorite, ethylene oxide, aldehydes, alcohol.
PHYSICAL METHODS: The use of this method and its effectiveness depends on two factors: exposure time and temperature, especially when it comes to using heat. All micro-organisms are susceptible, to varying degrees, to the action of heat. The heat causes denaturing of proteins, fusion and disorganization of membranes and/or irreversible oxidizing processes in microorganisms.
Other physical ways to sterilize are moist heat, autoclave, dry heat, stoves – ovens, radiation even to ultraviolet rays.
However, there is one that hasn’t been talked about much yet, and that is ultrasonic waves, a product that you can find in Kalstein. This method removes microorganisms quite efficiently without damaging any surface or material subjected to this cleaning. The way an ultrasonic cleaner works is using cavitation bubbles in a high frequency pressure waves (sound) that agitates a liquid. When this happens, a force on contaminants is produced in surfaces such as metals, plastics, glass, rubber, and ceramics. By doing this, you can also clean blind holes, cracks, and recesses.
Remember that it is always ideal to document yourself until you get the perfect method for your lab.